murine sv40 transfected mesangial cell line sv40 mes 13  (ATCC)

Journal: Oncotarget

Article Title: A novel pan-Nox inhibitor, APX-115, protects kidney injury in streptozotocin-induced diabetic mice: possible role of peroxisomal and mitochondrial biogenesis

doi: 10.18632/oncotarget.18540

Figure Lengend Snippet: Diabetes was induced in mice by intraperitoneal injection of STZ (50 mg/kg). Then APX-115 (60 mg/kg/day) or losartan (1.5 mg/kg/day) was administered orally for 12 weeks to diabetic mice. After 12 weeks, urine and blood samples were collected for analysis of ( A ) urinary albumin excretion, ( B ) albumin/creatinine ratio, ( C ) creatinine clearance rate, and ( D ) plasma cystatin C. ( E ) Kidneys were fixed in paraffin and cut into 3 μm sections that were subsequently stained with PAS reagent. Scale bar: 10 μm; original magnification: 630×. After PAS staining, ( F ) glomerular volume, ( G ) mesangial area, and ( H ) tuft area were analyzed using Image-Pro Plus 4.5.1. DM, STZ-induced diabetic mice. Data are presented as means ± SE of 10–12 mice/group; * p < 0.05 vs. control, † p < 0.05 vs. DM.

Article Snippet: A murine mesangial cell line (MES-13, cloned from mice transgenic for the early region of SV-40 virus, passage 25) was obtained from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Injection, Clinical Proteomics, Staining, Control

Journal: Oncotarget

Article Title: A novel pan-Nox inhibitor, APX-115, protects kidney injury in streptozotocin-induced diabetic mice: possible role of peroxisomal and mitochondrial biogenesis

doi: 10.18632/oncotarget.18540

Figure Lengend Snippet: ( A ) Plasma LPO, ( B ) urinary LPO, ( C ) kidney tissue LPO, ( D ) Nox1, ( E ) Nox2, and ( F ) Nox4 mRNA expression levels in kidneys were measured using real-time PCR. ( G and H ) Frozen kidney sections were stained with DHE at 5 µM (original magnification: 400×; scale bar: 20 μm). (A–H) Data are presented as means ± SE of 10–12 mice/group; * p < 0.05 vs. control, † p < 0.05 vs. DM. ( I ) Mesangial cells were incubated with or without APX-115 (1 µM) for 30 min and stimulated with or without 30 mM high glucose (HG) for 24 h followed by angII for 30 min. After that cells were incubated with 10 µM DCF-DA for 10 min and the fluorescence intensity was measured with a Zeiss vision system. Data are presented as means ± SE of at least 2 independent experiments; * p < 0.05 vs. control, † p < 0.05 vs. angII or angII+HG in DMSO.

Article Snippet: A murine mesangial cell line (MES-13, cloned from mice transgenic for the early region of SV-40 virus, passage 25) was obtained from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Clinical Proteomics, Expressing, Real-time Polymerase Chain Reaction, Staining, Control, Incubation, Fluorescence

Journal: Scientific Reports

Article Title: Involvement of Endoplasmic Reticulum Stress, Autophagy, and Apoptosis in Advanced Glycation End Products-Induced Glomerular Mesangial Cell Injury

doi: 10.1038/srep34167

Figure Lengend Snippet: Mesangial cells treated with AGEs (10, 20, 40, 80 and 160 μg/ml) for 24 h. BSA (160 μg/ml) was used as a negative control. The cell viability was evaluated by MTT assay ( A ). For apoptosis evaluation, cells were stained with Annexin V and PI after treated with AGEs. Cells were treated with 3% formaldehyde for 30 minutes as a positive control (PC). Percentages of apoptotic cells were determined by flow cytometry ( B ). Data are presented as mean ± SEM of three independent experiments performed in triplicates. * P < 0.05 as compared to control group. # P < 0.05 as compared to BSA-treated group.

Article Snippet: Murine glomerular mesangial cell line (SV40 MES13) was purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan).

Techniques: Negative Control, MTT Assay, Staining, Positive Control, Flow Cytometry, Control

Journal: Scientific Reports

Article Title: Involvement of Endoplasmic Reticulum Stress, Autophagy, and Apoptosis in Advanced Glycation End Products-Induced Glomerular Mesangial Cell Injury

doi: 10.1038/srep34167

Figure Lengend Snippet: Mesangial cells were transfected with single Atg5 siRNA [(MSS247019; siRNA (1), (A-a)] or mixture of Atg5 siRNAs [(MSS247019, MSS247020, and MSS247021; siRNA (2), (A-b)] or single Atg5 siRNA [(MSS247020; siRNA (3), (B-a)] or scramble control before AGEs treatment. Cells were treated with BSA (160 μg/ml) or AGEs (160 μg/ml) for 24 h. The protein levels of Atg5, p62, LC3, phospho-eIF2α, CHOP, and cleaved caspase-3 were determined by Western blot ( A ). The percentages of apoptotic cells were determined by PI-Annexin V staining ( B ). Data are presented as mean ± SEM of three independent experiments performed in duplicates ( A ) or triplicates ( B ). * P < 0.05 as compared to BSA-treated group or AGEs-treated group.

Article Snippet: Murine glomerular mesangial cell line (SV40 MES13) was purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan).

Techniques: Transfection, Control, Western Blot, Staining

Journal: Journal of Food and Drug Analysis

Article Title: Myrciaria cauliflora extracts attenuate diabetic nephropathy involving the Ras signaling pathway in streptozotocin/nicotinamide mice on a high fat diet

doi: 10.1016/j.jfda.2015.10.001

Figure Lengend Snippet: Myrciaria cauliflora extract (MCE) decreases mesangial matrix expansion in the glomerulus. (A) Kidney slices were embedded in paraffin, stained with hematoxylin and eosin. Light photomicrographs (400× magnification) of kidney sections from control group (a), streptozotocin/nicotinamide (STZ/NA) group (b), STZ/NA + 0.1% MCE group (c), STZ/NA + 0.5% MCE group (d), and STZ/NA + 1.0% MCE group (e). Arrow indicates white circle, the location of mesangial matrix expansion in the glomerulus. (B) Quantification of the mesangial matrix area. Three randomly selected glomeruli per mouse were examined. Data are shown as mean ± standard deviation; n = 10 mice per group. † p < 0.05 versus control group; * p < 0.05, ** p < 0.001 versus STZ/NA group.

Article Snippet: A murine kidney mesangial cell line (MES13) was obtained from BCRC (Bioresource Collection and Research Center, Hsinchu, Taiwan) and grown in a 3:1 mixture of Dulbecco’s modified Eagle medium and Ham’s F-12 medium containing 5% (v/v) fetal bovine serum, 4mM L-glutamine, 1mM penicillin/streptomycin, and 5.5mM glucose.

Techniques: Staining, Control, Standard Deviation